matlab imbinarize function Search Results


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imbinarize matlab function  (MathWorks Inc)
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Imbinarize Matlab Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imbinarize function  (MathWorks Inc)
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Imbinarize Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imbinarize.m matlab function  (MathWorks Inc)
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functions imbinarize and bwhitmiss  (MathWorks Inc)
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adaptive threshold matlab function imbinarize  (MathWorks Inc)
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Adaptive Threshold Matlab Function Imbinarize, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab's 'graythresh' function  (MathWorks Inc)
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Quantification of filament numbers and lengths. (A) Flow-chart summarizing image processing and quantification steps. (B) Representative micrograph of filaments polymerized from 2 µM actin monomers, labeled with FITC-phalloidin, diluted into microscopy buffer, and imaged using TIRF microscopy. (C-E) Micrograph shown in B following sequential (C) noise filtering and background subtraction, (D) normalization and image <t>thresholding,</t> and (E) binarization and skeletonization. (F) (Left) Following image processing, overlapping filaments are detected as errors and highlighted in red. (Right) Each misidentified filament is processed individually until all errors are resolved, and all filaments are highlighted in green. (G) Histogram of filament lengths measured for the representative micrograph shown in panel B. The bin size is 3 µm.
Matlab's 'graythresh' Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab function imbinarize  (MathWorks Inc)
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Quantification of filament numbers and lengths. (A) Flow-chart summarizing image processing and quantification steps. (B) Representative micrograph of filaments polymerized from 2 µM actin monomers, labeled with FITC-phalloidin, diluted into microscopy buffer, and imaged using TIRF microscopy. (C-E) Micrograph shown in B following sequential (C) noise filtering and background subtraction, (D) normalization and image <t>thresholding,</t> and (E) binarization and skeletonization. (F) (Left) Following image processing, overlapping filaments are detected as errors and highlighted in red. (Right) Each misidentified filament is processed individually until all errors are resolved, and all filaments are highlighted in green. (G) Histogram of filament lengths measured for the representative micrograph shown in panel B. The bin size is 3 µm.
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contrast enhancing filter  (MathWorks Inc)
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Quantification of filament numbers and lengths. (A) Flow-chart summarizing image processing and quantification steps. (B) Representative micrograph of filaments polymerized from 2 µM actin monomers, labeled with FITC-phalloidin, diluted into microscopy buffer, and imaged using TIRF microscopy. (C-E) Micrograph shown in B following sequential (C) noise filtering and background subtraction, (D) normalization and image <t>thresholding,</t> and (E) binarization and skeletonization. (F) (Left) Following image processing, overlapping filaments are detected as errors and highlighted in red. (Right) Each misidentified filament is processed individually until all errors are resolved, and all filaments are highlighted in green. (G) Histogram of filament lengths measured for the representative micrograph shown in panel B. The bin size is 3 µm.
Contrast Enhancing Filter, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Quantification of filament numbers and lengths. (A) Flow-chart summarizing image processing and quantification steps. (B) Representative micrograph of filaments polymerized from 2 µM actin monomers, labeled with FITC-phalloidin, diluted into microscopy buffer, and imaged using TIRF microscopy. (C-E) Micrograph shown in B following sequential (C) noise filtering and background subtraction, (D) normalization and image thresholding, and (E) binarization and skeletonization. (F) (Left) Following image processing, overlapping filaments are detected as errors and highlighted in red. (Right) Each misidentified filament is processed individually until all errors are resolved, and all filaments are highlighted in green. (G) Histogram of filament lengths measured for the representative micrograph shown in panel B. The bin size is 3 µm.

Journal: Biology Open

Article Title: Computational tools for quantifying actin filament numbers, lengths, and bundling

doi: 10.1242/bio.060267

Figure Lengend Snippet: Quantification of filament numbers and lengths. (A) Flow-chart summarizing image processing and quantification steps. (B) Representative micrograph of filaments polymerized from 2 µM actin monomers, labeled with FITC-phalloidin, diluted into microscopy buffer, and imaged using TIRF microscopy. (C-E) Micrograph shown in B following sequential (C) noise filtering and background subtraction, (D) normalization and image thresholding, and (E) binarization and skeletonization. (F) (Left) Following image processing, overlapping filaments are detected as errors and highlighted in red. (Right) Each misidentified filament is processed individually until all errors are resolved, and all filaments are highlighted in green. (G) Histogram of filament lengths measured for the representative micrograph shown in panel B. The bin size is 3 µm.

Article Snippet: A thresholding algorithm (implemented using MATLAB's ‘graythresh’ and ‘imbinarize’ functions ( https://www.mathworks.com/help/images/ref/graythresh.html ; https://www.mathworks.com/help/images/ref/imbinarize.html ) is then applied to convert detected objects from a grayscale into binary images ( ).

Techniques: Labeling, Microscopy

Kinetic measurements of filament bundling. Filaments were assembled from 2 µM actin monomers, labeled with FITC-phalloidin, and visualized by TIRF microscopy following the addition of 1.4 µM fascin. (A) Time series of micrographs depicting the progress of filament bundling. The same micrographs are also shown in Figure 4 to demonstrate the effects of image segmentation on the resolution of filaments and bundles. (B) Micrograph collected at time=0 s, before bundling has occurred. Following background subtraction, noise filtering, thresholding and skeletonization, the image has been segmented into a grid. The numbers correspond to the threshold fluorescence value (reported in arbitrary units of fluorescence intensity) above which pixels are considered bundled within each segment of the grid. (C) Automated detection of stretches of bundled (yellow) and single (magenta) filaments in each of the micrographs shown in panel A. (D) The fraction of the filamentous actin that is bundled over time for the reaction shown in panel A.

Journal: Biology Open

Article Title: Computational tools for quantifying actin filament numbers, lengths, and bundling

doi: 10.1242/bio.060267

Figure Lengend Snippet: Kinetic measurements of filament bundling. Filaments were assembled from 2 µM actin monomers, labeled with FITC-phalloidin, and visualized by TIRF microscopy following the addition of 1.4 µM fascin. (A) Time series of micrographs depicting the progress of filament bundling. The same micrographs are also shown in Figure 4 to demonstrate the effects of image segmentation on the resolution of filaments and bundles. (B) Micrograph collected at time=0 s, before bundling has occurred. Following background subtraction, noise filtering, thresholding and skeletonization, the image has been segmented into a grid. The numbers correspond to the threshold fluorescence value (reported in arbitrary units of fluorescence intensity) above which pixels are considered bundled within each segment of the grid. (C) Automated detection of stretches of bundled (yellow) and single (magenta) filaments in each of the micrographs shown in panel A. (D) The fraction of the filamentous actin that is bundled over time for the reaction shown in panel A.

Article Snippet: A thresholding algorithm (implemented using MATLAB's ‘graythresh’ and ‘imbinarize’ functions ( https://www.mathworks.com/help/images/ref/graythresh.html ; https://www.mathworks.com/help/images/ref/imbinarize.html ) is then applied to convert detected objects from a grayscale into binary images ( ).

Techniques: Labeling, Microscopy, Fluorescence